Part:BBa_K5371004
mCherry
mCherry is a red fluorescent protein (RFP) consisting of 236 amino acids with a molecular weight of 26.7 kDa. It is widely used as a reporter gene in molecular biology due to its high photostability, making it suitable for long-term in vivo and in vitro studies. The protein’s fluorescence allows for real-time tracking of gene expression, transfection efficiency, and the spatial-temporal dynamics of cellular processes. The chromophore-binding domain, which gives mCherry its red fluorescence, is primarily due to three crucial amino acids: tyrosine72, glycine73, and methionine71. After translation, these residues undergo post-translational modification, forming imidazoline groups. Upon illumination with UV light, a second oxidation step creates an acylimine linkage, resulting in red emission. As a reporter, mCherry is often used to label genes, cells, or organelles of interest. Its fluorescent properties make it a powerful tool for studying constitutive gene expression and intracellular processes.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 349
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 349
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 349
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 349
- 1000COMPATIBLE WITH RFC[1000]
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